Description
GAPDH Polyclonal Antibody | E-AB-40337 | Gentaur UK, US & Europe Distribution
Type: Control Antibody
Synonyms: aging associated gene 9 protein, BARS-38, cb609, G3PDH, GAPD, KNC-NDS6, OCAS, p38 component
Application: WB, IHC
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Reserch Areas: Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism
Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.
Concentration: 0.66 mg/mL
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Immunogen: Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Buffer: PBS with 0.05% Proclin300, 50% glycerol, pH7.3
Purification Method: Antigen Affinity Purification
Dilution: WB 1:3000-1:6000 IHC 1:300-1:500
Clone: N/A
Conjugation: Unconjugated
Molecular Weight(Calculated): 35 kDa
Molecular Weight(Observed): 35 kDa