GEMC1 Antibody | 6385

GEMC1 Antibody | 6385 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: GEMC1 antibody was raised against a 19 amino acid synthetic peptide near the center of human GEMC1.
The immunogen is located within amino acids 130 - 180 of GEMC1.

Research Area: Cancer, Cell Cycle

Tested Application: E, WB

Application: GEMC1 antibody can be used for detection of GEMC1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1401 - Mouse Heart Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: GEMC1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: GEMC1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: GEMC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: GEMC1 Antibody: GEMC1, GEMC1, Geminin coiled-coil domain-containing protein 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: GEMC1 Antibody: The Geminin coiled-coil domain-containing protein 1 (GEMC1) is a highly conserved protein that is required for chromosomal replication. GEMC1 contains a region similar to the coiled-coil domain of geminin, an essential vertebrate replication protein in which the coiled-coil domain is required for the function. GEMC1 binds to the checkpoint and replication factor TopBP1, allowing the binding of GEMC1 to chromatin during pre-replication complex formation. GEMC1 also directly interacts with replication factors Cdc45 and Cdk2-CyclinE and stimulates the initiation of DNA replication. Depletion of GEMC1 by siRNA in embryonic and somatic vertebrate cells prevents DNA replication due to impairment of Cdc45 loading onto chromatin, suggesting GEMC1 promotes initiation of DNA replication by mediating TopBP1 and Cdk2 dependent recruitment of Cdc45 onto replication origins.