General 1, 5-AG (1, 5-Anhydroglucitol) ELISA Kit | ELK7840

General 1, 5-AG (1, 5-Anhydroglucitol) ELISA Kit | ELK7840 | Gentaur UK, US & Europe Distribution

Alternative Names: LycoMark; 1-Deoxy-D-glucose; 1-Deoxy-D-glucopyranose; 1, 5-Anhydro-D-glucitol; 1, 5-Anhydrosorbitol; Aceritol; Polygalytol

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.6 μg/mL

Standard: 150 μg/mL

Detection Range: 2.35-150 μg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Nutrition metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1, 5-Anhydroglucitol (1, 5-AG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1, 5-Anhydroglucitol (1, 5-AG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1, 5-Anhydroglucitol (1, 5-AG) in the samples is then determined by comparing the OD of the samples to the standard curve.