General 12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit | ELK8156

General 12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit | ELK8156 | Gentaur UK, US & Europe Distribution

Alternative Names: 12HETE

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 5.32 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 12-Hydroxyeicosatetraenoic Acid (12-HETE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 12-Hydroxyeicosatetraenoic Acid (12-HETE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 12-Hydroxyeicosatetraenoic Acid (12-HETE) in the samples is then determined by comparing the OD of the samples to the standard curve.