General 5-HETE (5-Hydroxyeicosatetraenoic Acid) ELISA Kit | ELK8155

General 5-HETE (5-Hydroxyeicosatetraenoic Acid) ELISA Kit | ELK8155 | Gentaur UK, US & Europe Distribution

Alternative Names: 5-HETE

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 3.95 ng/mL

Standard: 800 ng/mL

Detection Range: 12.5-800 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Enzyme & Kinase

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxyeicosatetraenoic Acid (5-HETE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxyeicosatetraenoic Acid (5-HETE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxyeicosatetraenoic Acid (5-HETE) in the samples is then determined by comparing the OD of the samples to the standard curve.