General 5-HIAA (5-Hydroxyindoleacetic Acid) ELISA Kit | ELK7838

General 5-HIAA (5-Hydroxyindoleacetic Acid) ELISA Kit | ELK7838 | Gentaur UK, US & Europe Distribution

Alternative Names: 5HIAA

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 1.93 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: serum, plasma, urine, cerebrospinal fluid and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Tumor immunity;Endocrinology;Urology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxyindoleacetic Acid (5-HIAA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxyindoleacetic Acid (5-HIAA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxyindoleacetic Acid (5-HIAA) in the samples is then determined by comparing the OD of the samples to the standard curve.