General 5-Me-THF (5-Methyltetrahydrofolate) ELISA Kit | ELK8059

General 5-Me-THF (5-Methyltetrahydrofolate) ELISA Kit | ELK8059 | Gentaur UK, US & Europe Distribution

Alternative Names: 5-Me-THFA; 5-Me-H4FA; 5-Me-THF; 5-Me-H4F; Levomefolic Acid; Metafolin; 5-Methyltetrahydrofolic Acid; L-Methylfolate

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.43 ng/mL

Standard: 90 ng/mL

Detection Range: 1.41-90 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Hematology;Reproductive science;Nutrition metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Methyltetrahydrofolate (5-Me-THF) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Methyltetrahydrofolate (5-Me-THF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Methyltetrahydrofolate (5-Me-THF) in the samples is then determined by comparing the OD of the samples to the standard curve.