General 8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit | ELK7861

General 8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit | ELK7861 | Gentaur UK, US & Europe Distribution

Alternative Names: 8OHdG; 7, 8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7, 8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 7, 8-dihydrodeoxyguanosine

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 26.29 pg/mL

Standard: 6000 pg/mL

Detection Range: 93.75-6000 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-Hydroxydeoxyguanosine (8-OHdG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-Hydroxydeoxyguanosine (8-OHdG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-Hydroxydeoxyguanosine (8-OHdG) in the samples is then determined by comparing the OD of the samples to the standard curve.