General AP (Allopregnanolone) ELISA Kit | ELK7894

General AP (Allopregnanolone) ELISA Kit | ELK7894 | Gentaur UK, US & Europe Distribution

Alternative Names: THP; 3a, 5a-Tetrahydro Progesterone

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.55 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Endocrinology;Reproductive science;Genetic science;Nutrition metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Allopregnanolone (AP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Allopregnanolone (AP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Allopregnanolone (AP) in the samples is then determined by comparing the OD of the samples to the standard curve.