General CH (Cholesterol) ELISA Kit | ELK8282

General CH (Cholesterol) ELISA Kit | ELK8282 | Gentaur UK, US & Europe Distribution

Alternative Names: CH

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 2.48 μg/mL

Standard: 500 μg/mL

Detection Range: 7.82-500 μg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cholesterol (CH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cholesterol (CH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholesterol (CH) in the samples is then determined by comparing the OD of the samples to the standard curve.