855

General DAG (Diacylglycerol) ELISA Kit | ELK8147

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SKU:
855-ELK8147-GEN
€1,629.00 - €1,905.00

Description

General DAG (Diacylglycerol) ELISA Kit | ELK8147 | Gentaur UK, US & Europe Distribution

Alternative Names: DG; Diglyceride

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 141 pg/mL

Standard: 100000 pg/mL

Detection Range: 1562.5-100000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Signal transduction;Endocrinology;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Diacylglycerol (DAG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Diacylglycerol (DAG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diacylglycerol (DAG) in the samples is then determined by comparing the OD of the samples to the standard curve.

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