General DHT (Dihydrotestosterone) ELISA Kit | ELK7979

General DHT (Dihydrotestosterone) ELISA Kit | ELK7979 | Gentaur UK, US & Europe Distribution

Alternative Names: 5α-DHT; 5α-Dihydrotestosterone; Androstanolone; Stanolone

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 13.3 pg/mL

Standard: 2500 pg/mL

Detection Range: 39.07-2500 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Endocrinology;Reproductive science;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dihydrotestosterone (DHT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dihydrotestosterone (DHT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dihydrotestosterone (DHT) in the samples is then determined by comparing the OD of the samples to the standard curve.