General KYNA (Kynurenic Acid) ELISA Kit | ELK8136

General KYNA (Kynurenic Acid) ELISA Kit | ELK8136 | Gentaur UK, US & Europe Distribution

Alternative Names: Kinurenic acid; Kynuronic acid; Kuinurenic acid; Transtorine

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 9.24 ng/mL

Standard: 2000 ng/mL

Detection Range: 31.25-2000 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Enzyme & Kinase

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Kynurenic Acid (KYNA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kynurenic Acid (KYNA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kynurenic Acid (KYNA) in the samples is then determined by comparing the OD of the samples to the standard curve.