Description
General LTC4 (Leukotriene C4) ELISA Kit | ELK8118 | Gentaur UK, US & Europe Distribution
Alternative Names: LT-C4
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 142.5 pg/mL
Standard: 30000 pg/mL
Detection Range: 468.75-30000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Metabolic pathway;Infection immunity
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Leukotriene C4 (LTC4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Leukotriene C4 (LTC4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leukotriene C4 (LTC4) in the samples is then determined by comparing the OD of the samples to the standard curve.