General NTM (Natamycin) ELISA Kit | ELK8241

General NTM (Natamycin) ELISA Kit | ELK8241 | Gentaur UK, US & Europe Distribution

Alternative Names: Pimaricin; Natacyn

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 1.45 ng/mL

Standard: 300 ng/mL

Detection Range: 4.69-300 ng/mL

Sample Type: biological agents

Assay Lenght: 2h

Research Area: Infectious Diseases

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Natamycin (NTM) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Natamycin (NTM) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Natamycin (NTM) in the samples is then determined by comparing the OD of the samples to the standard curve.