General RVT (Resveratrol) ELISA Kit | ELK8265

General RVT (Resveratrol) ELISA Kit | ELK8265 | Gentaur UK, US & Europe Distribution

Alternative Names: trans-3, 5, 4'-Trihydroxystilbene; 3, 4', 5-Stilbenetriol; trans-Resveratrol

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 89.3 ng/mL

Standard: 20000 ng/mL

Detection Range: 312.5-20000 ng/mL

Sample Type: plant tissue and biological fluids

Assay Lenght: 2h

Research Area: Cardiocerebral vascular system

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Resveratrol (RVT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Resveratrol (RVT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Resveratrol (RVT) in the samples is then determined by comparing the OD of the samples to the standard curve.