General SPH (Sphingomyelin) ELISA Kit | ELK8171

General SPH (Sphingomyelin) ELISA Kit | ELK8171 | Gentaur UK, US & Europe Distribution

Alternative Names: SM

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 1.85 μg/mL

Standard: 400 μg/mL

Detection Range: 6.25-400 μg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Signal transduction;Neuro science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sphingomyelin (SPH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sphingomyelin (SPH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingomyelin (SPH) in the samples is then determined by comparing the OD of the samples to the standard curve.