General VD3 (Vitamin D3) ELISA Kit | ELK8102

General VD3 (Vitamin D3) ELISA Kit | ELK8102 | Gentaur UK, US & Europe Distribution

Alternative Names: Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 2.14 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Reproductive science;Nutrition metabolism;Bone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vitamin D3 (VD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitamin D3 (VD3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D3 (VD3) in the samples is then determined by comparing the OD of the samples to the standard curve.