Genomic column DNA Express (CE) | K-1-1/E

Genomic column DNA Express | K-1-1/E is available for delivery

Description:

General information: Genomic DNA from whole blood, plasma, buffy coat, body fluids, etc.

Target Disease Type: RNA&DNA purification

Specific Application: Spin Column Purification Method

Storage and Shipping :

Genomic column DNA Express (CE) K-1-1/E DataSheet

INTENDED USE

Kit Genomic Column DNA Express is designed for the rapid isolation of highly pure genomic DNA from whole blood, serum, plasma, cultured cells or other body fluids. It is also possible to purify viral DNA (e.g. HBV, CMV, EBV) from serum or plasma.

Genomic Column DNA Express is intended as general-purpose device. The kits allow purification of highly pure genomic DNA in less than 10 min with an A260/280-ratio between 1.60 and 1.90 and a typical concentration of 80 – 120 ng per µl.

PRINCIPLE OF ASSAY

Kit Genomic Column DNA Express is designed for genomic DNA extraction from whole blood, cultured cells, serum, plasma or other body fluids. Lysis is achieved by incubation of whole blood in a solution containing large amounts of chaotropic ions in the presence of proteinase K. Appropriate conditions for binding of DNA to the silica membrane of the corresponding columns are created by addition of ethanol to the lysate. The binding process is reversible and specific to nucleic acids.

Contaminations are removed by only a single wash step. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer. The procedure, which is ideal for simultaneous processing of multiple samples, yield pure DNA ready for direct amplification in just 10 minutes. The procedure is suitable for use with fresh or frozen whole blood and blood which has been treated with citrate, heparin, or EDTA.