GITRL Antibody | 36-221

GITRL Antibody | 36-221 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: Recombinant human GITRL.

Research Area: Cancer, Immunology

Tested Application: E, WB

Application: ELISA: (direct and indirect: 1:5, 000-1:10, 000) . Western Blot: (1:2, 000-1:5, 000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

Specificiy: Recognizes human GITRL. Detects a band of ~15-17kDa by Western blot. Does not cross-react with mouse GITRL.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Concentration: 1 mg/ml

Storage Condition: Stable for at least 6 months after receipt when stored at -20˚C.

Alternate Name: Glucocorticoid-induced TNF Receptor Ligand; AITRL; Activation-inducible TNF-related Ligand; TNFSF18

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: GITRL is expressed on dendritic cells (DC) , monocytes, macrophages, B cells, activated T cells, endothelial cells, osteoclasts and various healthy non-lymphoid tissues (e. g. testis, . ) . GITRL is constitutively expressed and released as soluble form by solid tumors and various hematopoietic malignancies. GITRL causes differentiation of osteoclasts, activation of macrophages, but also alteration of carcinoma and leukemia cells and influences apoptosis. Binding to GITR is important in regulating T-cell proliferation and TCR-mediated apoptosis. GITRL is implicated in development of autoimmune diseases and in the immune response against infectious pathogens and tumors.