Glutamine Synthetase, human recombinant | P1067

GLUL also known as Glutamine synthetase. It is a trimetallic enzyme containing two divalent cation sites and one monovalent cation site per subunit. GLUL is able to regulate intracellular concentrations of glutamate and catalyzes the synthesis of glutamine form glutamate and ammonia. It is ubiquitously expressed in the human and plays a major role for many metabolic pathways such as cell proliferation, inhibition of apoptosis, and cell signaling. Recombinant Human GLUL was expressed in E.coli and purified by using conventional chromatography techniques.

P1067 | Glutamine Synthetase human recombinant DataSheet

Biomolecule/Target: GLUL

Synonyms: GLNS, GS, PIG43, PIG59, GLUL

Alternates names: Apolipoprotein E4, APOE, apolipoprotein, apolipoproteins

Taglines: N/A

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: Human

Accession #:

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to convert L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.

Biological activity: N/A

Results: > 2.000 pmol/min/ug

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that convert L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH

Molecular Weight: 34.4 kDa

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: In 20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol 1 mM DTT, 0.1 mM PMSF

Reconstitution Instructions: Reconstitute in dHO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A