Glyoxalase I protein | 30R-1262

Glyoxalase I protein | 30R-1262 |

Activity Code: ACTIVE

Product Type: Proteins

Product Subtype: Recombinant Proteins

Research Area: Proteases, Inhibitors, & Enzymes

Short Description: Purified recombinant Human Glyoxalase I protein

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: Human

Residues: 1-184 amino acids: MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM

Tag/conjugate: N/A

Protein Type: Recombinant

Expression System: E.coli

Grade & Purity: > 90% pure

Methode of Purification: N/A

Source: N/A

Concentration: 1 mg/ml

Form & Buffer: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, and 10% glycerol.

Storage: Store at 4 deg C for short term storage (1/2 weeks) . Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping Information: *Blue Ice*

Applications: SDS-PAGE

Bioactivity: Specific activity: >0.4 units/mg (please enquire for specific batch value) . One unit will form 1.0 umol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 7.5, at 25 deg C.

Usage Recommendations: N/A

Assay Information: 1. Prepare a 1.5ml reaction mix into a suitable container: The final concentrations are 79 mM potassium phosphate, 0.033% (w/v) reduced glutathine, 0.003% (w/v) bovine serum albumin. 2. Equilibrate to 25 DEG C and monitor at A240nm (absorbance) until the value is constant, using a spectrophotometer. 3. Add 50ul of recombinant glyoxalase I solution with various concentrations (0.5ug, 1ug, 2ug) in 1.4ml reaction buffer. 4. Immediately mix by inversion and record the increase at A240nm for 5 minutes.