GM-CSF, Porcine Recombinant | P1561

GM-CSF, also known as granulocyte macrophage colony-stimulating factor, is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Recombinant porcine GM-CSF was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

P1561 | GM-CSF, Porcine Recombinant DataSheet

Biomolecule/Target :

Synonyms: Colony stimulating factor, Colony Stimulating Factor 2, Colony stimulating factor 2 (granulocyte-macrophage), Colony-stimulating factor, CSF, CSF 2, CSF2, CSF2_HUMAN, GM-CSF, GMCSF

Alternates names: Colony stimulating factor, Colony Stimulating Factor 2, Colony stimulating factor 2 (granulocyte-macrophage), Colony-stimulating factor, CSF, CSF 2, CSF2, CSF2_HUMAN, GM-CSF, GMCSF

Taglines: A cytokine that stimulates the bone marrow to produce granulocytes and stem cells

NCBI Gene ID #: 397208

NCBI Gene Symbol: CSF2

Gene Source: Porcine

Accession #: Q29118

Recombinant: TRUE

Source: E. coli

Purity by SDS-PAGE: >95%

Assay: SDS - PAGE

Purity:

Assay #2:

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Activity (Specifications/test method): Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 40 ng/ml.

Biological activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 40 ng/ml.

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: 16.6 kDa (His tag at N-terminal)

Concentration:

Appearance: Liquid

Physical form description: Liquid

Reconstitution Instructions:

Amino acid sequence: aa 148 (aa 18-144)

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans