Description
GMCSF protein | 30R-1055 |
Activity Code: ACTIVE
Product Type: Proteins
Product Subtype: Recombinant Proteins
Research Area: Cytokines & Growth Factors
Short Description: Purified recombinant Human GMCSF protein
Immunogen: N/A
Host: N/A
Specificity: N/A
Cross Reactivity: N/A
Isotype: N/A
IClone: N/A
Species: Human
Residues: MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE
Tag/conjugate: N/A
Protein Type: Recombinant
Expression System: E.coli
Grade & Purity: > 95% pure
Methode of Purification: N/A
Source: N/A
Concentration: 1 mg/ml
Form & Buffer: Supplied as a liquid in PBS, pH 7.4.
Storage: Store at 4 deg C for short term storage (1/2 weeks) . Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping Information: *Blue Ice*
Applications: SDS-PAGE
Bioactivity: The ED50 for this effect is < 0.18 ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
Usage Recommendations: N/A
Assay Information: Cell line: TF1 (human erythroleukemic cell) . Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF Assay medium: 10% FBS RPMI 1640 without Gm-CSF Cell density: 2 x 10, 000 cells/well (96 well plate, final volume 100ul) Starvation: 24hr, Assay medium Incubation time: 24 hr (after sample treatment) Concentration range: 0.048 ng/ml - 25 ng/ml Detection method: MTT assay