GMCSF protein | 30R-1055

GMCSF protein | 30R-1055 |

Activity Code: ACTIVE

Product Type: Proteins

Product Subtype: Recombinant Proteins

Research Area: Cytokines & Growth Factors

Short Description: Purified recombinant Human GMCSF protein

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: Human

Residues: MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

Tag/conjugate: N/A

Protein Type: Recombinant

Expression System: E.coli

Grade & Purity: > 95% pure

Methode of Purification: N/A

Source: N/A

Concentration: 1 mg/ml

Form & Buffer: Supplied as a liquid in PBS, pH 7.4.

Storage: Store at 4 deg C for short term storage (1/2 weeks) . Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping Information: *Blue Ice*

Applications: SDS-PAGE

Bioactivity: The ED50 for this effect is < 0.18 ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.

Usage Recommendations: N/A

Assay Information: Cell line: TF1 (human erythroleukemic cell) . Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF Assay medium: 10% FBS RPMI 1640 without Gm-CSF Cell density: 2 x 10, 000 cells/well (96 well plate, final volume 100ul) Starvation: 24hr, Assay medium Incubation time: 24 hr (after sample treatment) Concentration range: 0.048 ng/ml - 25 ng/ml Detection method: MTT assay