Description
GNAS Antibody | 42-594 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human, Mouse, Rat
Homology: Expected Species Reactivity based on sequence homology: Cow
Immunogen: The immunogen for this antibody is: C-QAARSNSDGEKATK
Research Area: Cancer
Tested Application: E, WB
Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 45kDa band observed in lysates of cell line Jurkat (calculated MW of 45.7kDa according to NP_000507.1) . Recommended concentration: 1-3ug/ml. Approx. 50kDa band observed in lysates of Mouse and Rat Brain (calculated MW of 45.6kDa according to NP_963910.1) . Recommended concentration: 0.1-0.3ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Specificiy: This antibody is expected to recognize all three reported isoforms NP_000507.1, NP_001070956.1 and NP_536350.2. However, in Mouse it is expected to recognize reporterd isoforms GNASL (NP_963910.1) and XLas (NP_034439.2) only.
Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: GNAS, GNAS complex locus, AHO, C20orf45, GNAS1, GPSA, GSA, GSP, MGC33735, NESP, PHP1A, PHP1B, POH, dJ309F20.1.1, dJ806M20.3.3, OTTHUMP00000031742, OTTHUMP00000196026, OTTHUMP00000196030, adenylate cyclase-stimulating G alpha protein, guanine nucleotide binding protein (G protein) , alpha stimulating activity polypeptide 1, guanine nucleotide regulatory protein, neuroendocrine secretory protein, secretogranin VI
User Note: N/A
BACKGROUND: N/A