Goat anti-Human IgG Fc, HRP conjugated, min, reactivity to human IgA and IgM | AS10 664

Goat anti-Human IgG Fc, HRP conjugated, min, reactivity to human IgA and IgM | AS10 664 | Gentaur UK, US & Europe Distribution

Immunogen: affinity purified human IgG Fc part

Host: Goat

Conjugation: HRP

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified goat IgG.

Format: Lyophilized

Tested Application: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Related Products: antibodies to human IgG

Recommended Dilutions: 1 : 10 000 -1 : 100 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 100 000 (WB)

Molecular weight: N/A

Confirmed Reactivity: Human IgG heavy chain Fc based on immunoelectrophoresis

Predicted Reactivity: Human IgG Fc

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: No reactivity to serum proteins from bovine or rabbit, No reactivity is observed to human IgA or IgM based on immunoelectrophoresis

Background: Goat anti-human IgG Fc is a secondary antibody conjugated to HRP which binds to human Fc part of IgG in immunological assays. The antibody is absorbed agains human IgA and IgM

Reconstitution: For reconstitution add 1, 1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1, 1 ml of sterile water add 1, 1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

TAIR Nnumbre: N/A

Category: IgG

Research Area: Immunoassays, Immunology