Goat Anti-Rat IgG (H&L) Antibody (Alkaline Phosphatase) | XR-9580

Goat Anti-Rat IgG (H&L) Antibody (Alkaline Phosphatase) | XR-9580 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Rat

Immunogen: Rat IgG - whole molecule

Tested Application: E, WB, IHC

Application: Alkaline Phosphatase Conjugated Affinity Purified anti-Rat IgG is suitable for immunoblotting (western or dot blot) , ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:4,000 - 1:20,000, Western Blot 1:1,000 - 1:5,000, Immunohistochemistry 1:200 - 1:1,000 . (Optimal dilution should be determined by user) .

Specificiy: Minimum Cross reactivity to Human Serum Proteins

Purification: Alkaline Phosphatase Conjugated Affinity Purified anti-Rat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) , anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Human Serum Proteins.

Isotype: IgG

Conjugate: Alkaline Phosphatase

Physical State: Liquid

Buffer: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 0.01% (w/v) Sodium Azide; 10 mg/mL Bovine Serum Albumin (BSA)

Concentration: N/A

Storage Condition: Alkaline Phosphatase Conjugated Affinity Purified anti-Rat IgG can be stored vial at 4 ˚C before opening. DO NOT FREEZE. This product is stable at 4 ˚C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.

Target Species: Reacts with the heavy chain of rat IgG.