Goat anti-Rat IgG (H&L), HRP conjugated, min, cross-reactivity to human, mouse lgG (highly absorbed against mouse IgG) | AS09 618

Goat anti-Rat IgG (H&L), HRP conjugated, min, cross-reactivity to human, mouse lgG (highly absorbed against mouse IgG) | AS09 618 | Gentaur UK, US & Europe Distribution

Immunogen: Purified Rat IgG, whole molecule

Host: Goat

Conjugation: HRP

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified goat IgG.

Format: Lyophilized

Tested Application: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Related Products: AS09 619 | Goat anti-rat IgG (H&L) biotin conjugatedAS06 620 | Goat anti-rat IgG (H&L) ALP (alkaline phosphatase) conjugated

Recommended Dilutions: 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)

Molecular weight: N/A

Confirmed Reactivity: Rat IgG heavy and light chains (H&L) of all Rat immunoglobulins

Predicted Reactivity: Rat IgG Heavy and Light chains (H&L) of all Rat immunoglobulins

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: No reactivity is observed to non-immunoglobulin rat serum, Antibody has been absorbed against mouse IgG and no reactivity is observed to human or mouse IgG based on immunoelectrophoresis

Background: Goat anti-rat IgG is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.

Reconstitution: For reconstitution add 1, 1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily

Storage: Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

TAIR Nnumbre: N/A

Category: HRP (horse radish peroxidase)

Research Area: Immunoassays