Goat Total cholesterol (TC) ELISA Kit | ELK8796

Goat Total cholesterol (TC) ELISA Kit | ELK8796 | Gentaur UK, US & Europe Distribution

Alternative Names: TC

Species: Goat

Assay Type: Competitive Inhibition

Sensitivity: 2.63 μg/mL

Standard: 500 μg/mL

Detection Range: 7.82-500 μg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TC (Total cholesterol ) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TC (Total cholesterol ) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TC (Total cholesterol ) in the samples is then determined by comparing the OD of the samples to the standard curve.