GOGAT | Glutamine oxoglutarate aminotransferase | AS07 242

GOGAT | Glutamine oxoglutarate aminotransferase | AS07 242 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide well conserved in known GOGAT sequences from different species including Arabidopsis thaliana Fd-GOGAT 1 Q9ZNZ7, At5g04140 and Fd-GOGAT 2 Q9T0P4, At2g41220

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: Collection of antibodies to proteins involved in nitrogen metabolismPlant and algal protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 1000 (WB)

Molecular weight: 177 | 170-180 kDa depending upon the species

Confirmed Reactivity: Arabidopsis thaliana, Miscanthus giganteus, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Populus tremula, Solanum lycopersicum, Spartina sp., Zea mays

Predicted Reactivity: Arthrospira platensis PCC 7345, Beta vulgaris, Brachypodium distachyon, Burkholderia glumae, Camellia sinensis, Chloroflexi bacterium, Chlamydonas reinhardtii, Gracilaria tenustipitata, Cyanobacteria, Crocosphaera watsonii WH 8502, Cyanidioschyzon merolae (strain 10D), Galdieria sulphuraria, Glycine max, Helicosporidium sp. ATCC 50920, Hydrogenobaculum sp. HO, Lactococcus lactis subsp. lactis bv. diacetylactis str. TIFN2, Leptospira interrogans serovar Bataviae str. HAI135, Leptolyngbya boryana, Medicago trucutula, Microcystis panniformis, Monoraphidium neglectum, Morus notabilis, Nannochloropsis gaditana, Nicotiana tabacum, Ostreococcus lucimarinus, Physcomitrium patens subsp. patens, Porphyra purpurea, Sutterella wadsworthensis, Sulfurihydrogenibium yellowstonense, Veillonella atypica Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: This antibody can be used as a plastidial marker for leaf material, not roots where levels of GOGAT are too low. A 40 kDa band present in A. thaliana sample is not competed away during antibody neutralization assay. In this assay free peptide used for antibody production is incubated together with anti-GOGAT antibodies. Due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time.

Background: Glutamine oxoglutarate aminotransferase (abbreviated as GOGAT) is an enzyme involved in synthesis of glutamate from glutamine and alpha-ketoglutarate. GOGAT has two forms in plants: ferredoxin-dependent GOGAT (Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT) . 95% of GOGAT found in plants is the Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 and glu2 found on chromosomes 5 and 2 respectively (in Arabidopsis) . Fd-GOGAT (both forms) is highly conserved among plants, red algae, and cyanobacteria.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT2G41220

Category: Global Antibodies

Research Area: Plant Biology