Description
GOT2, also known as glutamate oxaloacetate transaminase 2, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Glutamate oxaloacetate transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
7809 | GOT2 human recombinant DataSheet
Biomolecule/Target: GOT2
Synonyms: Aspartate aminotransferase 2, mitochondrial, KAT4, KATIV, mitAAT
Alternates names: Aspartate aminotransferase 2, mitochondrial, KAT4, KATIV, mitAAT
Taglines: Plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles.
NCBI Gene ID #: 26291
NCBI Gene Symbol: FGF21
Gene Source: Human
Accession #: Q9NSA1
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Specific activity is > 44 unit/mg, in which one unit will convert 1.0 µmole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25ºC.
Results: Specific activity is > 44 unit/mg
Binding Capacity: N/A
Unit Definition: One unit will convert 1.0 µmole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25ºC.
Molecular Weight: 48.4 kDa (433 aa, 1-413 aa + His Tag)
Concentration: 0.5 mg/ml
Appearance: Liquid
Physical form description: 0.5 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 150 mM NaCl and 1 mM DTT.
Reconstitution Instructions: N/A
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK