Gremlin-1, human recombinant | 7159

Gremlin-1 (isoform-1) belongs to a group of diffusible proteins which bind to ligands of the TGF- family and regulate their activity by inhibiting their access to signaling receptors. The interplay between TGF- ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule. Gremlin is highly expressed in the small intestine, fetal brain, and colon and lower expression in brain, prostate, pancreas and skeletal muscle. Gremlin-1 regulates multiple functions in early development by specifically binding to and inhibiting the function of BMP-2, -4, and -7. It also plays a role in carcinogenesis and kidney branching morphogenesis. Recombinant Gremlin-1 is an 18.3 kDa protein containing 160 amino acid residues.

7159 | Gremlin-1 human recombinant DataSheet

Biomolecule/Target: Gremlin-1

Synonyms: CKTSF1B1, DAND2, DRM, IHG-2

Alternates names: FST, FS, Activin-binding protein

Taglines: A TGF- antagonist

NCBI Gene ID #: 10468

NCBI Gene Symbol: FST

Gene Source: Human

Accession #: P19883

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 0.07-0.11 g/ml

Biological activity: Determined by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The expected ED is 0.1-0.4 µg/ml in the presence of 7.5 ng/ml Activin A.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 31.5 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5 and 150 mM NaCl.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A