GRF-1 (phospho-Y1105) polyclonal Antibody | BS4232

GRF-1 (phospho-Y1105) polyclonal Antibody | BS4232 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IHC

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200

Background: GRF-1 (glucocorticoid receptor DNA-binding factor 1), also known as p190RhoGAP or simply p190, is a transcriptional regulator which binds to the promoter region of the glucocorticoid receptor gene and represses its expression. By repressing GR expression, GRF-1 acts to down-regulate Rho signaling, thereby mediating both actin cytoskeletal rearrangements and cell cycle events. Through its GAP domain, GRF-1 is thought to affect cytokinesis by regulating Rho activity; a regulation that is controlled by the ubiquination of the GTP binding region and subsequent degradation of GRF-1. Additionally, GRF-1 plays an important role in oligodendrocyte differentiation, a process that is absent in malignant glioma tumors, implicating GRF-1 as a possible tumor suppressor. GRF-1 expression is regulated by glucocorticoids and the expressed protein exists as two isoforms produced by alternative splicing events.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p-GRF-1 (Y1105) polyclonal Antibody detects endogenous levels of GRF-1 protein only when phosphorylated at Tyr1105.

Molecular Weight: ~ 172 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Rho GTPase-activating protein 35; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-A; ARHGAP35; GRF1; GRLF1; KIAA1722

Immunogen: Synthetic phosphopeptide derived from human GRF-1 around the phosphorylation site of Tyrosine 1105.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Notch Signaling,Nuclear Receptor Signaling,Cell Cycle Control G1 S Checkpoint,Epigenetic Writers and Erasers of Histone H3,Epigenetic Writers and Erasers of Histones H2A H2B and H4,