GSK-3, human recombinant | 7004

Glycogen synthase kinase-3 (GSK-3) is a serine/theronine kinase that was first characterized by its role in intermediary metabolism. In mammals, serine kinase is encoded by two related genes called GSK-3 (51 kDa) and GSK-3 (46 kDa). GSK-3 has been implicated in the regulation of the c-Jun/AP-1 transcription factor and other intracellular signaling pathways.

7004 | GSK-3 human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: GSK, Glycogen synthase kinase-3 (GSK-3)

Alternates names: HO:HO oxidoreductase

Taglines: A phosphorylating and an inactivating agent of glycogen synthase

NCBI Gene ID #: 847

NCBI Gene Symbol: CAT

Gene Source: Human

Accession #: P04040

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): Activity of GSK-3 was determined by an in vitro kinase assay using 100 ng Tau as substrate and 1 µg of the recombinant GSK-3 in a 20 µl reaction containing 25 mM Tris-HCl, pH 7.5, 5 mM -glycerol phosphate, 12 mM MgCl, 2 mM DTT, 0.1 M Na3VO4, and 200 µM ATP. After 30 minutes incubation at 30°C, phosphorylation of Tau by the recombinant GSK-3 was determined by Western blot using a phospho-Tau antibody.

Biological activity:

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 244.0 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized from 2 mg/ml in 0.1 M NH4CO3

Reconstitution Instructions: N/A

Amino acid sequence: N/A