GSTA4, human recombinant | 7800

GSTA4, also known as glutathione S-transferase A4, belongs to the GST superfamily. This enzyme is involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. GSTA4 shows very high activity with reactive carbonyl compounds such as alk-2-enals. GSTA4 is highly effective in catalyzing the conjugate addition of reduced glutathione to 4-hydroxynonenal, an important product of peroxidative degradation of arachidonic acid and a commonly used biomarker for oxidative damage in tissue. This enzyme is expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver. Recombinant human GSTA4 protein, fused to His-tag at N-terminus, was expressed in E.Coli and purified by using conventional chromatography techniques.

7800 | GSTA4 human recombinant DataSheet

Biomolecule/Target: GSTA4

Synonyms: Glutathione S-transferase alpha 4, GSTA4-4, GTA4

Alternates names: Glutathione S-transferase alpha 4, GSTA4-4, GTA4

Taglines: Involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds

NCBI Gene ID #: 2323

NCBI Gene Symbol: Flt3-L

Gene Source: Human

Accession #: P49771

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity > 0.9 units/mg, one unit is defined as the amount of enzyme that conjugate 1.0 µmole of 1-chloro-2, 4- dinitrobenzene (CDNB), with reduced glutathione per minute pH 6.5 at 25ºC.

Results: Specific activity > 0.9 units/mg

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that conjugate 1.0 µmole of 1-chloro-2, 4- dinitrobenzene (CDNB), with reduced glutathione per minute pH 6.5 at 25ºC.

Molecular Weight: 28.3 kDa (246 aa, 1-222 aa + His Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 20% glycerol, 2 mM DTT and 100 mM NaCl.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAARPK LHYPNGRGRM ESVRWVLAAA GVEFDEEFLE TKEQLYKLQD GNHLLFQQVP MVEIDGMKLV QTRSILHYIA DKHNLFGKNL KERTLIDMYV EGTLDLLELL IMHPFLKPDD QQKEVVNMAQ KAIIRYFPVF EKILRGHGQS FLVGNQLSLA DVILLQTILA LEEKIPNILS AFPFLQEYTV KLSNIPTIKR FLEPGSKKKP PPDEIYVRTV YNIFRP