GSTM3, human recombinant | 7801

Glutathione S-transferase mu 3, also known as GSTM3, is member of the glutathione s-transferase (GST) family of proteins. There are eight families of GST proteins, namely alpha, kappa, mu, omega, pi, sigma, theta and zeta, each of which is composed of proteins that have a variety of functions throughout the cell. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTM3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

7801 | GSTM3 human recombinant DataSheet

Biomolecule/Target: GSTM3

Synonyms: Glutathione S-transferase mu 3, GST5, GSTB, GSTM3-3, GTM3

Alternates names: Glutathione S-transferase mu 3, GST5, GSTB, GSTM3-3, GTM3

Taglines: Functions in the detoxification of electrophilic compounds

NCBI Gene ID #: 2323

NCBI Gene Symbol: Flt3-L

Gene Source: Human

Accession #: P49771

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity > 4.7 units/mg, one unit is defined as the amount of enzyme that conjugate 1.0 µmole of 1-chloro-2, 4-dinitrobenzene (CDNB), with reduced glutathione per minute pH 6.5 at 25ºC.

Results: Specific activity > 4.7 units/mg

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that conjugate 1.0 µmole of 1-chloro-2, 4-dinitrobenzene (CDNB), with reduced glutathione per minute pH 6.5 at 25ºC.

Molecular Weight: 29.1 kDa (249 aa, 1-225 aa)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 10% glycerol, 1 mM DTT and 100 mM NaCl.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSCESS MVLGYWDIRG LAHAIRLLLE FTDTSYEEKR YTCGEAPDYD RSQWLDVKFK LDLDFPNLPY LLDGKNKITQ SNAILRYIAR KHNMCGETEE EKIRVDIIEN QVMDFRTQLI RLCYSSDHEK LKPQYLEELP GQLKQFSMFL GKFSWFAGEK LTFVDFLTYD ILDQNRIFDP KCLDEFPNLK AFMCRFEALE KIAAYLQSDQ FCKMPINNKM AQWGNKPVC