Guinea pig IgG2 (Immunoglobulin G2) ELISA Kit | ELK5991

Guinea pig IgG2 (Immunoglobulin G2) ELISA Kit | ELK5991 | Gentaur UK, US & Europe Distribution

Alternative Names: IGHG2; Ig Gamma-2 Chain C Region; Immunoglobulin Heavy Constant Gamma 2; G2m Marker; Immunoglobulin Gm2

Species: Guinea pig

Assay Type: Competitive Inhibition

Sensitivity: 124.5 ng/mL

Standard: 25000 ng/mL

Detection Range: 390.63-25000 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 2h

Research Area: Infection immunity;Immune molecule

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Immunoglobulin G2 (IgG2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immunoglobulin G2 (IgG2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G2 (IgG2) in the samples is then determined by comparing the OD of the samples to the standard curve.