H3 | Histone H3 (antigen affinity purified) | AS10 710A

H3 | Histone H3 (antigen affinity purified) | AS10 710A | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030, At4g40040, At5g10980), H3.2 P59226 (At1g09200, At3g27360, At5g10390, At5g10400, At5g65360), H3-like 2 Q9FXI7 (At1g19890)

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: ChIp-qPCR (ChIp-qPCR), Western blot (WB)

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Recommended Dilutions: 2, 5 µg/100 µg of chromatin (ChIp-qPCR), 1 : 5000 (WB)

Molecular weight: 15 | 17 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Chlamydomonas reinhardtii, Physcomitrium patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays Brachypodium distachyon, Brassica napus, Hordeum vulgare, Nicotiana tabacum, Malus domestica, Medicago sativa, Nannochloropsis gaditana, Triticum aestivum, Pinus pinaster, Pisum sativum, Oryza sativa, Zea mays, Vitis vinifera, Volvox sp. Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Protocol for isolation of cytosolic and nuclear fractions can be found here.Specific protol for ChIP can be found here: Saleh et al. (2008) .

Background: Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT4G40030

Category: Nuclear marker

Research Area: Cell Biology