Description
H3F3B Antibody | 13-512 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human H3F3B (NP_002098.1) .
Research Area: Other
Tested Application: WB, IF
Application: WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Specificiy: N/A
Positive Control 1: A-549
Positive Control 2: SW620
Positive Control 3: U-87MG
Positive Control 4: MCF7
Positive Control 5: LO2
Positive Control 6: N/A
Molecular Weight: Observed: 15kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: H3.3B, histone H3.3, H3 histone, family 3B (H3.3B)
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17.