H3K18me2 | Histone H3 (dimethylated Lys18) | AS16 3182

H3K18me2 | Histone H3 (dimethylated Lys18) | AS16 3182 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum.

Format: Liquid

Tested Application: Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Western blot (WB)

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Recommended Dilutions: 2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 500 (IF), 1 : 100 (IHC), 1 : 500 (WB)

Molecular weight: 15 kDa

Confirmed Reactivity: Human

Predicted Reactivity: Caenorhabditis elegans, Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: N/A

Background: The di-methylated K18 on histone H3 is a seemingly transient post-translational modification. H3K18 is better known to be acetylated, and occasionally mono-methylated. Suv39h1, a well-studied histone methyltransferase seems to be responsible for the transition of acetylation and methylation at this H3 modification site. The di-methylated K18 on H3 seems to be associated with embryological development and possibly implantation.

Reconstitution: N/A

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Epigenetics/DNA methylation

Research Area: Epigenetics