H3R17me2 (asym) | Histone H3 (asym-dimethyl Arg17) | AS16 3180

H3R17me2 (asym) | Histone H3 (asym-dimethyl Arg17) | AS16 3180 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum.

Format: Liquid

Tested Application: Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF), Western blot (WB)

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Recommended Dilutions: 2-5 µg/million cells (ChIP), 1 : 50 (IF), 1 : 500 (WB)

Molecular weight: 15 kDa

Confirmed Reactivity: Caenorhabditis elegans, Human

Predicted Reactivity: Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: N/A

Background: Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, dimetylation of H3 Arg17 (H3 R17Me2) has been linked to gene activation. Coactivator-associated arginine methyltransferase-1 (CARM1) methylates Arg17 with its protein arginine methyltransferase (PRMT) catalytic core. Activation of this modification is linked to transcription hormone response promotors, as well as cell fate regulation. Interestingly, H3 methylation of R17 and R26 contributes to greater pluripotency potential of stem cells, while downregulation of this PTM increases differentiation.

Reconstitution: N/A

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Epigenetics/DNA methylation

Research Area: Epigenetics