855

Hamster MMP19 (Matrix Metalloproteinase 19) ELISA Kit | ELK9518

(No reviews yet) Write a Review
SKU:
855-ELK9518-GEN
NULL588.00 - NULL704.00

Description

Hamster MMP19 (Matrix Metalloproteinase 19) ELISA Kit | ELK9518 | Gentaur UK, US & Europe Distribution

Alternative Names: RASI-1; MMP18; Occasionally Referred To As Stromelysin-4

Species: Hamster

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Tumor immunity;Reproductive science;Developmental science;Rheumatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 19 (MMP19) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Matrix Metalloproteinase 19 (MMP19) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 19 (MMP19), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 19 (MMP19) in the samples is then determined by comparing the OD of the samples to the standard curve.

View AllClose