HDAC-3, Human Recombinant | 7613

Human Histone Deacetylase 3 (HDAC3) is a member of the class I Histone Deacetylases. HDACs are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells. HDACs catalyze the removal of acetyl groups from lysines near the N-termini of histones. Human HDACs have been implicated in a variety of human diseases such as cardiomyopathy, osteodystrophy, neurodegenerative disorders, aging and cancer. Like other class I and II HDAC members, the activity of HDAC3 is sensitive to HDAC inhibitor Trichostatin A (TSA).

7613 | HDAC-3 human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: HDAC-3, HDAC3, HDAC 3, Histone deacetylase 3

Alternates names: IBP-1, IGF-Binding Protein 1, AFBP, PP12, IGF-BP25, hIGFBP-1, IGFBP-1.

Taglines: A potential tumor suppressor gene

NCBI Gene ID #: 3484

NCBI Gene Symbol: IBP1

Gene Source: Human

Accession #: P08833

Recombinant: Yes

Source: Sf9 insect cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 0.5 µU/µl

Biological activity: The ED as determined by its ability to inhibit IGF-I induced proliferation of NUC-1 cells is ~100 ng/ml.

Results: ~100 ng/ml

Binding Capacity: N/A

Unit Definition: One unit of the recombinant HDAC3 is defined as the amount of enzyme that deacetylates 1 pmol of substrate Arg-His-Lys-Lys(Ac)-AFC per minute at 37°C in a reaction solution containing 50 mM Tris, pH 7.5, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2 and 1 mg/ml BSA.

Molecular Weight: 28.8 kDa

Concentration: 0.5U/µl

Appearance: Liquid

Physical form description: rh-HDAC-3 is supplied with N-term His-tag as a liquid in 25 mM Tris, pH 7.5, 100 mM NaCl, 2.7 mM KCl, 3 mM MgCl2, 10 % glycerol.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A