Description
HDAC2 Antibody | 27-648 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse, Dog
Homology: N/A
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HDAC2.
Research Area: Transcription, Cancer
Tested Application: E, WB, IHC
Application: HDAC2 antibody can be used for detection of HDAC2 by ELISA at 1:12500. HDAC2 antibody can be used for detection of HDAC2 by western blot at 0.2-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Specificiy: N/A
Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 55 kDa
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by peptide affinity chromatography method.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration: batch dependent
Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store HDAC2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Alternate Name: HDAC2, HD2, RPD3, YAF1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Histone deacetylase 2 (HDAC2) , or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly concerved from yeast to human.