HDAC6, human recombinant | 7534

HDAC6 is a Class II HDAC. It is unique among human HDACs in having two full deacetylase domains. As a tubulin deacetylase, HDAC6 may act to promote autophagic clearing of Huntington aggregates and retard HIV1 infection. It can shuttle between the nucleus and cytoplasm, suggesting potential extra nuclear functions by regulating the acetylation status of non-histone substrates. By modifying chromatin structure and other non-histone proteins, HDACs play important roles in controlling complex biological events, including cell development, differentiation, programmed cell death, angiogenesis, and inflammation. Considering these major roles, it is conceivable that dysregulation of HDACs and subsequent imbalance of acetylation and deacetylation may be involved in the pathogenesis of various diseases, including cancer and inflammatory diseases.

7534 | HDAC6 human recombinant DataSheet

Biomolecule/Target: HDAC6

Synonyms: KIAA0901, JM21, HD6

Alternates names: KIAA0901, JM21, HD6

Taglines: Regulates the acetylation status of non-histone substrates

NCBI Gene ID #: 5340

NCBI Gene Symbol: PLG

Gene Source: Human

Accession #: P00747

Recombinant: Yes

Source: Baculovirus

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/g by LAL method

Activity (Specifications/test method): N/A

Biological activity: 560 pmole/min/g. Assay conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 M HDAC substrate (Cat # 2396), and HDAC6 (7.8 – 500 ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature.

Results: 560 pmole/min/g.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 159 kDa (N-terminal GST tag)

Concentration: N/A

Appearance: Liquid

Physical form description: In 50 mM Tris-HCl, pH 8.0, 500 mM NaCl, 50 mM KCl, 2 mM EDTA, 10% glycerol, and 2 mM DTT.

Reconstitution Instructions: N/A

Amino acid sequence: N/A