Heat Shock Protein 90 (Hsp90 beta) NT-His Tag, human recombinant | 4858H

Hsp90 is a molecular chaperone and is one of the most abundant proteins in unstressed cells. In mammalian cells, there are two genes encoding cytosolic Hsp90 homologues, with the human Hsp90 showing 85% sequence identity to Hsp90. The recombinant Hsp90 produced from Sf9 cells represents the full length (a.a. 1-723) of Hsp90 subunit and a N-term His-tag.

4858H | Heat Shock Protein 90 Hsp90 beta NT-His Tag human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Heat Shock Protein 90, HSP90

Alternates names: FS, activin-binding protein, FSH-suppressing protein (FSP)

Taglines: A chaperone protein that assists protein folding

NCBI Gene ID #: 14313

NCBI Gene Symbol: FST

Gene Source: Human

Accession #: P47931

Recombinant: Yes

Source: Sf9 cells

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The activity is determined by the dose-dependent neutralization of 7.5 ng/mL human Activin A. Complete neutralization is typically reached at less than 0.3 ug/mL.

Results: 0.3 ug/mL

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 31.6 kDa

Concentration: 5 mg/ml

Appearance: Liquid

Physical form description: 5mg/ml in 40 mM HEPES/KOH, pH 7.5, containing 20 mM potassium chloride and 5% glycerol

Reconstitution Instructions: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.

Amino acid sequence: MGNCWLRQAK NGRCQVLTKT ELSKEECCST GRLSTSWTEE DVNDNTLFKW MIFNGGAPNC IPCKETCENV DCGPGKKCRM NKKNKPRCVC APDCSNITWK GPVCGLDGKT YRNECALLKA RCKEQPELEV QYQGRCKKTC RDVFCPGSST CVVDQTNNAY CVTCNRICPE PASSEQYLCG NDGVTYSSAC HLRKATCLLG RSIGLAYEGK CIKAKSCEDI QCTGGKKCLW DS