Heat Shock Protein 90 (Hsp90), human recombinant | 4858

Hsp90 is a molecular chaperone and is one of the most abundant proteins in unstressed cells. In mammalian cells, there are two genes encoding cytosolic Hsp90 homologues, with the human Hsp90 showing 85% sequence identity to Hsp90. The recombinant Hsp90 produced from Sf9 cells represents the full length (a.a. 1-723) of Hsp90 subunit.

4858 | Heat Shock Protein 90 Hsp90 human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Heat Shock Protein 90, HSP90

Alternates names: NKSF, CLMF, Cytotoxic lymphocyte maturation factor 40 kDa subunit

Taglines: A chaperone protein that assists protein folding

NCBI Gene ID #: 16160

NCBI Gene Symbol: IL12

Gene Source: Human

Accession #: P43432

Recombinant: Yes

Source: Sf9 cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The activity is determined by the induced expression of CD25 on CD8+ mouse T cells and is typically detectable starting at 0.1 ng/mL.

Results: 0.1 ng/mL

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 70 kDa

Concentration: 5 mg/ml

Appearance: Liquid

Physical form description: 5mg/ml in 40 mM HEPES/KOH, pH 7.5, containing 400 mM potassium chloride and 5% glycerol

Reconstitution Instructions: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.

Amino acid sequence: p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS