Hexokinase 1 Antibody | 8217

Hexokinase 1 Antibody | 8217 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%)

Immunogen: Hexokinase 1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human Hexokinase 1.
The immunogen is located within amino acids 830 - 880 of Hexokinase 1.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: Hexokinase 1 antibody can be used for detection of Hexokinase 1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: Hexokinase 1 antibody is human, mouse and rat reactive. Multiple isoforms of Hexokinase 1 are known to exist.

Positive Control 1: Cat. No. 1478 - Rat Bladder Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 101 kDa
Observed: 103 kDa

Validation: N/A

Isoform: N/A

Purification: Hexokinase 1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Hexokinase 1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Hexokinase 1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: HK, HXK

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: There are four major glucose-phosphorylating isoenzymes, designated Hexokinase 1 I, II, III, and IV (1) . Hexokinase 1 activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway (1, 2) . Hexokinase 1 2 is the predominant Hexokinase 1 isozyme expressed in insulin-responsive tissues such as skeletal muscle and its expression is insulin-responsive (3) . It is involved in the increased rate of glycolysis seen in rapidly growing cancer cells (4) .