HINT1 Antibody [AMM02521G]

HINT1 Antibody [AMM02521G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H, M, Rat

Specificity: This HINT1 antibody is generated from a mouse immunized with a recombinant protein of human HINT1.

Cellular Localisation: Cytoplasm. Nucleus. Note=Interaction with CDK7 leads to a more nuclear localization.

Molecular Weight: 13802

Clone: Monoclonal

Gene Name: HINT1

Gene ID: 3094

Function: Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2 (PubMed:15703176, PubMed:16835243, PubMed:17337452, PubMed:17217311, PubMed:23614568, PubMed:28691797, PubMed:29787766, PubMed:31990367, PubMed:22329685) . Hydrolyzes adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate) (PubMed:15703176, PubMed:16835243) . Hydrolyzes lysyl-AMP (AMP-N-epsilon- (N-alpha-acetyl lysine methyl ester) ) generated by lysine tRNA ligase, as well as Met- AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon- (N-alpha-acetyl lysine methyl ester) ) and AMP-N-alanine methyl ester (PubMed:15703176, PubMed:22329685, PubMed:17337452) . Hydrolyzes 3-indolepropionic acyl- adenylate, tryptamine adenosine phosphoramidate monoester and other fluorogenic purine nucleoside tryptamine phosphoramidates in vitro (PubMed:17337452, PubMed:23614568, PubMed:29787766, PubMed:17217311, PubMed:28691797, PubMed:31990367) . Can also convert adenosine 5'-O- phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide (PubMed:30772266) . In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1 (PubMed:16014379, PubMed:22647378) . Modulates p53/TP53 levels and p53/TP53-mediated apoptosis (PubMed:16835243) . Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex (PubMed:19112177) . Also exhibits SUMO- specific isopeptidase activity, deconjugating SUMO1 from RGS17 (PubMed:31088288) . Deconjugates SUMO1 from RANGAP1 (By similarity) .

Summary: Tissue Location: Widely expressed.

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB, FC, IHC-P, IF

Dilution: IF--1:25 IHC-P--1:100~500 FC--1:25 WB--1:4000

Synonyms: Histidine triad nucleotide-binding protein 1, 3---, Adenosine 5'-monophosphoramidase, Protein kinase C inhibitor 1, Protein kinase C-interacting protein 1, PKCI-1, HINT1, HINT, PKCI1, PRKCNH1